Variants prolactin

Sinha, Y. N. (1995). Structural Variants of Prolactin Occurrence and Physiological Significance. Endocrine Reviews 16(3), 354—369. 

Hydrophilic polymeric support materials provide alternatives to silica for the preparation of HlC sorbents. These materials are more stable to high-pH conditions used as part of the clean in place (CIP) regimes [46]. Representative examples of HIC sorbents are contained in Table 3, and many other in-house laboratory variants can be identified in the scientific literature. From the list of commercialized HIC sorbents found in Table 3, it is apparent that the majority of preparative HIC sorbents can be obtained in particle size ranges (typically 20-65 pm), pore size distributions (typically = 600-1000 A), and surface chemistries suitable for large-scale applications with proteins and peptides. The TSK PS copolymer materials or the Pharmacia cross-linked Superose/agarose range of HIC sorbents are well-known examples in industry. Illustrative of the various uses of silica- and polymer-based HIC sorbents are the examples of avian lysozymes [144], human pituitary prolactin [275], recombinant subtilisin variants [145], and other globular proteins [276-278],... 

Sinha, Y.N. (1995) Structural variants of prolactin occurence and physiological significants. Endocrine Reviews, 16, 354-369. 

Endocrine A study of 47 boys found that prolactin levels were significantly associated with oral risperidone dose and risperidone and 9-hydroxyrisperidone levels, but not the TaqlA variant in the dopamine D2 receptor gene or variants of the CYP2D6 gene [259 ]. 

Protein isoforms result from PTMs, splicing variants, etc. Each protein isoform has its own pi and values 2DGE, or 2DGE coupled with corresponding protein antibodies, is an effective method to array those different isoforms of each protein. MS, especially MS/MS, plays an important role in the characterization of each PTM and splicing variant. We found that prolactin had multiple isoforms in human pituitary control tissues—six 2D gel spots that contained prolactin were identified [32 Evans et al., in preparation]. Further experiments are needed to determine whether the ratio of each prolactin isoform changes in NF pituitary adenomas and prolactinomas. 

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