Varian Inova instrument

NMR spectra were obtained on a Varian INOVA 400 instrument, at 400 MHz, in CDCI3. Flash chromatography was carried out with 230-400 mesh silica gel. 

NMR spectroscopy was conducted on both a Broker Avance 500 and a Varian Inova 400 instrument. Chemical shift as parts-per-million (ppm) on the 5-scale and coupling constants (J) in Hz were used throughout. Unless stated otherwise, all NMR-spectra were recorded in CDCI3 at 30°C with TMS as internal standard. The following... 

Elemental analyses were performed on a LECO CHNS 932 instrument. H-NMR spectra were determined with a Varian UNITY INOVA 500 MHz NMR spectrometer. IR spectra were recorded as KBr disks in the range of400 000 cm" on a SHIMADZU FTIR-8300 spectrometer. The electronic absorptions spectra were measiued in DMSO for 3,4 with a UNICAM UV2-100 UVATSIBLE spectrometer. 

Fig. 3. 2Q-HoMQC spectrum of apo-cytochrome c in 93% H2O at pH = 6 and 0.48 mM concentration. The spectrum was acquired with 30 ms 2Q excitation delay overnight on a Varian Unity/INOVA 600 MHz instrument using gradient MQ selection and no additional water suppression (I.P., unpublished). Equal spectral windows were set for both dimensions.

Figure 3 presents an example of such a situation. The 2Q-HoMQC spectrum of apo-cytochrome c was acquired in 93% H2O at 480 ixM concentration on a Varian Unity/INOVA 600 MHz NMR instrument overnight, using a pulse sequence with gradient coherence selection and weak gradient spin-echo during excitation delays and the evolution period [29], respectively (I.P., not published). The spectral windows were 8 kHz both in F2 and F. ... 

A Varian Unity Inova 600-MHz NMR instrument (Palo Alto, CA) equipped with a H C/ N pulse field gradient triple resonance microliow NMR probe (flow cell 60pL 3mm O.D.) was used. Reversed-phase HPLC of the samples was carried out on a Varian modular HPLC system (a 9012 pump and a 9065 photodiode array UV detector). The Varian HPLC software was also equipped with the capability for programmable stop-flow experiments based on UV peak detection. An LCQ classic MS instrument, mentioned in the previous section, was connected on-line to the HPLC-UV system of the LC-NMR by contact closure. The H resonance of the D2O was used for field-frequency lock, and the spectra were centered on the ACN methyl resonance. Suppression of resonances from HOD and methyl of ACN and its two C satellites was accomplished using a train of four selective WET pulses, each followed by a Bo gradient pulse and a composite 90-degree read pulse [41]. 

A Varian Unity Inova 600 MHz NMR instrument (Palo Alto, CA), equipped with a H C/ N pulse field gradient triple resonance microflow NMR probe (flow cell 60 pL 3 mm O.D.), was used. Reverse-phase HPLC of the samples was carried out with a Varian modular HPLC system (a 9012 pump and a 9065 photodiode array UV detector). The Varian HPLC software was also... 

The H NMR spectra were recorded using a Varian Unity Inova-300 instrument. The H chemical shifts are reported in 8 units (ppm) relative to tetramethylsilane (TMS) at the external standard (8 = 0.00). 

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