Using the Capacity Factor to Optimize Resolution

Effect of a change in kg on resolution and retention time. The original value of kg is assumed to be 1. 

The relationship between capacity factor and analysis time can be advantageous when a separation produces an acceptable resolution with a large b. In this case it may be possible to decrease b with little loss in resolution while significantly shortening the analysis time. 

Adjusting the capacity factor to improve resolution between one pair of solutes may lead to an unacceptably long retention time for other solutes. For example, improving resolution for solutes with short retention times by increasing 

The general elution problem in chromatography. Improving the resolution of the overlapping bands in chromatogram (a) results in a longer analysis time for chromatogram (b). 

The process of changing the column s temperature to enhance the separation of both early and late eluting solutes. 

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