Use of batch culture experiments

One simple, rapid approach to the problem is to perform a batch experiment and ensure that the initial substrate concentration S0, is very much greater than the 

This shows that a plot of In X against t will have a slope equal to nm. The experiment, therefore, simply involves making measurements of the biomass concentration X at a series of times during the growth phase. 

Gates and Marlar 0) devised a graphical procedure to determine the kinetic constants which took into account the data in the Anal stages of the growth-phase of a batch fermentation. As a result, their procedure can be used to estimate values of fim, Ks and the yield coefficient Y. The method involves rewriting equation 5.125, which is the complementary expression to the integrated form of the Monod equation 5.124, but refers to the substrate  

From this equation it may be seen that a plot of - In against — In (a + 1), (Fig. 

When the optimum value of is obtained the kinetic parameters can be calculated from the relationships  

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