Urine glyceric acid

Rashed, M.S. et al., Chiral liquid chromatography tandem mass spectrometry in the determination of the configurations of glyceric acid in urine of patients with D-glyceric and L-glyceric acidurias, Biomed. Chromatogr, 16, 191, 2002. 

Glyceric Add. Isherwood et al. (13) found glyceric acid in an amount of 2.6 mg/day in the urine of normal rats. Glyceric acid was also found in normal human urine by Osteux and Laturaze (06). 

It is impossible to distinguish these two types of oxaluria without careful biochemical investigation of the blood and the urine, and without direct attempts to identify the enzyme defect. The crucial difference between glycolic and glyceric aciduria is precisely that in addition to oxalate, glycolic acid accumulates in primary oxaluria, whereas glyceric acid accumulates in the second type [106, 107]. 

Vleigenthart, J. F. G., Duran, M., Ketting, D., and Wadman, S. K. Determination of the Configuration of Lactic and Glyceric Acids from Human Serum and Urine by Capillary Gas-Liquid Chromatography J. Chromatogr. 143(2) 117-123 (1977) CA 86 102229z... 

Fig. 4.1 Chromatogram of (9-acetylated menthyl esters of organic acids separated on a WCOT glass capillary column (25 mxO.3 mm i.d.) coated with SP-1000, under isothermal conditions (temperatures in parentheses) (a) synthetic / 5-lactic acid (150°C) (b) synthetic / 5-glyceric acid (200°C) (c) / -lactic acid isolated from urine (150°C) and (d) / -glyceric acid isolated from urine (200°C). (Redrawn with modifications from Kamerling etal.y 1977)...

Twenty-four-hour or spot urine should be collected in the presence of HC1. Add 10 ml of 20% (v/v) HC1 per liter of urine. Keep samples frozen at -20°C. Before injection into the HPLC system, dilute the urine (1) for oxalate 0.2 ml of centrifuged (3000 xg for 5 min) urine with 1.8 ml of 0.3 M boric acid (see below) (2) for glycolate and glycerate 0.2 ml of centrifuged (3000 xg for 5 min) urine with 1.8 ml of water. 

Following the load, approximately 4% of the test dose is excreted as D-glycerate in a 24-h urine. A loading test with 200-300 mg/kg of the amino acid L-serine may be equally effective. 

Dietzen DJ, Wilhite TR, Kenagy DN, Milliner DS, Smith CH, Landt M Extraction of glyceric and glycolic acids from urine with tetrahydrofuran utility in detection of primary hyperoxaluria. Clin Chem 1997 43 1315-1320. 

Fig. 14.8 Chromatogram of organic acids extracted using DEAE-Sephadex from urine of one of the patients (T.B.) described by Chalmers et al. (1980) (day after admission). Separated as their ethoxime and trimethylsilyl derivatives on 10 per cent OV-101 on HP Chromosorb W (80-100 mesh) by temperature programming from 110°C to 285°C at 4°C min with a 5 min initial isothermal delay. Peak identifications are 1, sulphate, 2, 3-hydroxybutyrate 3, acetoacetate 4, 3-hydroxyisovalerate 5, octanoate 6, phosphate 7, glycerate 8, 5-hydroxyhexanoate 9, 2-deoxytetronate 10, adipate 11, methyladipate 12, tetronates 13, 3-hydroxy-3-methylglutarate 14, 4-...

Fig. 16.2 Chromatogram of organic acids extracted using ethyl acetate and diethyl ether from the urine of a patient with o-glyceric aciduria (Brandt et al, 1976) and separated as their trimethylsiiyl derivatives on 3 per cent OV-1. Peak identifications are 1, glycollate plus lactate 2, urea 3, glycerate (d) 4, 4-hydroxyphenylacetate. (Redrawn with modifications from Brandt et al, 1976)...

Fig. 16.5 Chromatogram of organic acids extracted using DEAE-Sephadex from the urine of a patient with,primary hyperoxaluria type II, separated as described in the legend to Fig. 16.4. Peak identifications are 1, lactate plus some glycollate 2, oxalate 3, sulphate 4, phosphate 5, glycerate (l) 6, tetronates 7, citrate 8, n-tetracosane (standard) 9, n-hexacosane (standard). (The horizongtal axis represents the time elapsed in minutes from sample injection.)...

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