Urine analysis iodine speciation

Michalke and Schramel (1999) reported a method of CE coupled to ICP-MS for the speciation analysis of iodine. A buffer comprising phosphate (pH 2.3), NaOH, sodium dodecyl sulfate (SDS) and borate (pH 8.3) for stacking was employed for the separation of iodide, iodate, T4 and T3. The separated four iodine species were subsequently detected during a pressure-driven detection step (basehne-separated) at 19.5, 29.1, 36.6 and 42.2 s. The detection fimits were determined at 0.08 p,g I/l (iodide), 0.3 p.g I/l (iodate), 3.5 p.g I/l (T4) and 2.5 p,g I/l (T3). This method has been appfied for iodine speciation in human serum and urine. The serum from a healthy person contained iodide (13p.g I/l), T4 (6lp,g I/l) and T3 (7.5p.g I/l), whereas the serum from a thyroid-operated person lacked T3. 

Speciation analysis of iodine in hydrolyzed solution of tissues, urine and serum... 

Speciation analysis of iodine in human urine samples has also been carried out by ion chromatography (IC) combined with ICP-MS (Stark etai, 1997). Because of the possible interconversion of the iodine species, depending on the pH value, different eluent—column combinations were used for acidic or alkaline sample solutions. Iodide, iodate and several unidentified, presumably organo-iodine species could be separated and detected. 

In 1980s, Rack and colleagues at University of Nebraska carried out a series study on speciation of iodine, chlorinated pesticides, and seleium in urine using liquid chromatography and molecular neutron activation analysis. They developed procedures for trace level determination of iodoamino acids and... 

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