Uridine 3 -phosphate disodium salt

C9HiiN2Na209P 4H20 Uridine 3 -phosphate, disodium salt, tetra-hydrate ... 

Nucleosides, Nucleotides, Derivatives, and Related Compounds. — 5-Substituted uracils were examined for conformational dependence on the substituents, uridine 5 -phosphate disodium salt, 2 -deoxyuridine 5 -phosphate disodium salt, " 6-methyl-2 -deoxyuridine, 5-C-acetyl-2 -deoxyuridine, dihydrouridine 3 -phosphate potassium salt, 5-hydroxymethyl-2 -deoxyuridine, 4-thio--uridine, 2,5 -anhydro-2, 3 -0-isopropylidene-2-thiouracil, 2,2 -an-hydro-1 - 3-D-arabinofuranosyl-2-thiouracil. ... 

Prepare individual standards (Sigma) of adenosine-5 -phosphoric acid, cytidine 5 -phosphate (sodium salt), guanosine-5 -phosphate (sodium salt), and uridine-5 -phosphate (disodium salt) as l-pg/p. solutions in deionized water. Prepare each standard by dissolving 10 mg of the nucleotide in 10 ml of water. Prepare a mixed standard of the four nucleotides at 1 Ag/)il for each. To obtain the mixed standard, weigh 10 mg of each nucleotide and dissolve in 10 ml of water to achieve a concentration of 1 )ig/ J.l for each. 

The following separations (approximate values included) will be achieved for both individual nucleotides and the mixed standard guanosine-5 -phosphate (sodium salt), 0.34 adenosine-5 -phosphoric acid, 0.48 cytidine-5 -phosphate (sodium salt), 0.60 and uridine-5-phosphate (disodium salt), 0.74. The compounds will appear as dark spots against a bright fluorescent background at 254 nm. 

On silylation-amination of the disodium salts of inosine-5 -phosphate 238a or of guanosine-5 -phosphate 238 b with benzylamine, the phosphate moieties are also transiently protected during amination by silylation (cf also the silylation of uridine-5 -phosphate 224) to give, after transsilylation with methanol and addition of NaOH, the desired sodium salt of N -benzyladenosine-5 -phosphate 239a in 80% yield and the sodium salt of the 2-amino derivative 239 b in 78% yield [64] (Scheme 4.23). 

---