Urease metallochaperone

Colpas GJ, Brayman TG, Ming L-J, Hausinger RP. 1999. Identification of metalbinding residues in the Klebsiella aerogenes urease nickel metallochaperone, UreE. Biochemistry 38 4078-88. 

Three Ni-confaintng enzymes (see Nickel Enzymes Cofactors) appear to utilize Ni metallochaperones for enzyme activation. UreE appears to function in NP+ delivery to urease. The Klebsiella aerogenes protein binds 6 Ni per dimer, whereas that from Bacillus pasteurii binds a single Ni per dimer. The metal content differences arise from a His-Asp-His sequence near the middle and a histidine-rich region at the carboxyl terminus of the former protein. Truncated K aerogenes UreE protein, missing the His-rich... 

Song HK, Muleooney SB, Hubee R and Hau-siNGEE RP (2001) Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation. J Biol Chem 276 49359-49364. 

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