Unselective protein import

Unselective protein import. There is no barrier to the successful import of any precursor protein, nor to its processing and assembly into a functional, mature form. 

Estimating tolerance is important in predicting the potential for directed evolution. As a method of determine the average tolerance over an entire protein sequence (designability) rather than tolerance at specific positions. Suzuki et al. (1996) compared the distribution of mutations in selected and unselected libraries. If the protein was tolerant to substitutions, the number of mutations in both libraries would be the same. The average number of mutations for the selected library is lowered by the fraction that is deleterious. Indeed, they found that the O-helix of Taq polymerase I (Taq Pol I) is significantly less tolerant than the /33//34 region of HIV reverse transcriptase (HIV RT) (Fig. 17). They attribute this difference in survival rate versus number of mutations to... 

Models are constructed in analytics to describe the relationship between responses and factors. This is, for example, important for optimization of analytical methods on the basis of response surface methods (cf. Section 4.2). Models are also needed for cahbration of analytical methods. There, calibration of a single analyte in dependence on one or several wavelengths might be of interest. If, in the first example, the straight-line model would be adequate, for the second task of multiwavelength spectroscopy, multivariate approaches are needed. Calibrations in the case of unselective analytical methods must also be performed. These methods are termed simultaneous multicomponent analysis. In near-infrared (NIR) spectroscopy, the contents of water and protein in whole grain wheat are determined that way. 

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