Ultraviolet radiation light boxes

Photolysis is carried out in an 850-ml. Pyrex glass vessel equipped with a water-cooled quartz probe. The ultraviolet light source is a 450-watt lamp with a Vycor filterf. To reduce the dangers from a possible explosion or eye damage from ultraviolet radiation, the reaction vessel shown in Fig. 5 is contained in a wooden box. 

The Calcein-calcium complex fluoresces in ultraviolet radiation, but the uncomplexed Calcein does not. The sample to be titrated is placed in a dark room or in a box and exposed to uv radiation. The sample then is titrated until the fluorescence disappears. This is a very sharp endpoint and must be approached slowly near the end. The color of the fluorescence is enhanced by viewing though an amber glass or a piece of yellow cellophane, which removes the small amounts of visible blue light from the lamp. 

Preliminary experiments, using a container with reflective surfaces, employed light from eight fluorescent bulbs that produced a preponderance of radiation at 310 nm. Myristicin increased in samples that were irradiated 1 h and then kept for a 24-h induction period. However, heat produced by this system and the resulting dehydration of carrot roots may have affected results. Later experiments, employing a Chromato-Vue box used for ultraviolet examination of TLC plates, avoided these problems. One-hour light... 

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