Ultraviolet absorption resin elution

Filtered broth was passed at 2.5 ml/min through a resin column (2.5 cm diameter, 28 cm length) packed with 150 ml of ion exchange resin Amberlite IRC-50 sodium type (Rohm and Haas Co., U.S.A.). The column was washed with water, eluted with 0.5 N HCI at a flow rate 1.3 ml/min. The eluates were fractionated each 10 ml and tuberactinomycin-N activity was found at fractions No. 45-63 obsarved by ultraviolet absorption method and bioassay. 

DOWE) 50W-X4 sulfonic acid and DOWE) S) 1-X4 quaternary ammonium resins were eluted consecutively with 3 N NaOH, hot water, methanol, cold water, 3 N HCl, hot water, methanol, and cold water at least four times. The purity of the resins was checked by measuring the surface tension, conductance, ultraviolet absorption, and amount of acid or base required for neutralization of the wash water. The resins were converted to the and OH" forms, respectively, and mixed. 

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