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Ultrathin electrophoresis

A. Gerstner, Z. Csapo, M. Sasvari-Szekely, and A. Guttman, Ultrathin sodium dodecyl sulfate gel electrophoresis of proteins Effect of gel composition and temperature on the separation of sodium dodecyl sulfate-protein complexes, Electrophoresis, 21, 834 (2000). [Pg.718]

Moio, L., Di Luccia, A., and Addeo, F. (1988). Fast isoelectric focusing on small ultrathin polyacrylamide gels containing urea. Electrophoresis 10, 533-535. [Pg.209]

Membrane-mediated sample loading provides a rugged, easy loading mechanism for ultrathin-layer electrophoresis gels, conveniently applicable for both vertical and horizontal formats [11]. The samples are spotted manually or automatically (robots) onto the surface of the loading membrane tabs, outside of the separation platform. The sample spotted membrane is then placed into the injection (cathode) side of the separation cassette, in intimate contact with the gel edge. By the application of the electric field, the sample components migrate into the gel. There is no need to... [Pg.1672]

Fig. 1 Block diagram of the automated ultrathin-layer agarose gel electrophoresis system. Fig. 1 Block diagram of the automated ultrathin-layer agarose gel electrophoresis system.
Mutation Screening by Ultrathin-Layer Agarose Electrophoresis... [Pg.1673]

Zheng J, Odake T, Kitamori T, Sawada T. Miniaturized ultrathin slab gel electrophoresis with thermal lens microscope detection and its application to fast genetic diagnosis. Anal Chem 1999 71 5003-8. [Pg.140]

Guttman A, Ronai Z. Ultrathin-layer gel electrophoresis of biopolymers. (Review) Electrophoresis 2000 21 ... [Pg.140]

Hemoglobin variants have been typed by conventional electrophoresis on cellulose acetate or agarose and by lEF (1, 52, 66-70). Budowle and Eberhardt (5 ) recently developed an ULPAGIF method for typing the A, F, S, C and a number of rare variants, which is presently used for the analysis of bloodstained evidence submitted to the FBI Laboratory. The method employs pH 6.7-7.7 Pharmalytes in an ultrathin-layer gel with an inter-electrode wick distance of only 5.0 cm. The result is a rapid screening method for Hb that takes only 25-30 minutes-comparable to cellulose acetate. Further, the distances between the A-F, F-S and S-C were 4 oim, 7 mm and 12 mm, respectively, compared with 3 mm, 5.5 nim, and... [Pg.154]

Fig. 1 shows the block diagram of the automated ultrathin-layer agarose gel electrophoresis system consisting of a high-voltage power supply (1) platinum electrodes (2 and 3) ... [Pg.2376]

MUTATION SCREENING BY ULTRATHIN-LAYER AGAROSE ELECTROPHORESIS... [Pg.2377]

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See also in sourсe #XX -- [ Pg.53 ]



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