Ultrathin cryosection

Nielsen, M.H., Bastholm, L., Chatterjee, S., Koga, J., and Norrild, B. (1989) Simultaneous triple-immunogold staining of virus and host cell antigens with monoclonal antibodies of virus and host cell antigens in ultrathin cryosections. Elistochemistry 92, 89-93. [Pg.1098]

Frey B, Brunner I, Walther P, Scheidegger C, Zierold K. Element localization in ultrathin cryosections of high-pressure frozen ectomycorrhizal spruce roots. Plant Cell Environ 1997 20 929-937. [Pg.290]

Slot, J. W. and Geuze, H. J. (1984) Gold markers for single and double immuno-labeling of ultrathin cryosections, in Immunolabelling for Electron Microscopy (Polak, J. M. and Vamdell, I. M., eds.), Elsevier, New York, pp. 129-142. [Pg.353]

Chicoine, L., and Webster, P. 1998. Effect of microwave irradiation on antibody labeling efficiency when applied to ultrathin cryosections through fixed biological material. Microsc. Res. Tech. 42 24-32. [Pg.311]

Takizawa, T., Suzuki, K., and Robinson, J. M. (1998) Correlative microscopy using FluoroNanogold on ultrathin cryosections proof of principle. J. Histochem. Cytochem. 46, 1097-1102. [Pg.91]

J., Hyttinen, M.M., Jurvelin, J.S., and Helminen, HJ., Reference sample method reduces the error caused by variable cryosection thickness in Fourier transform infrared imaging, Appl. Spectrosc. 58, 137-140, 2004 Takizawa, T. and Robinson, J.M., Thin is better Ultrathin cryosection immunocytochemistry, J. Nippon Med. Sch. 71, 306-307, 2004. [Pg.86]

Fig. 14. Localization of PhoE-LacZ hybrid proteins in Escherichia coli by means of cell fractionation studies (A) showing clearly the presence of the hybrid protein in the total cellular proteins of induced cells (lane e), the cell envelopes of induced cells (lane f) and the Triton X-100 insoluble protein fraction of the cell envelopes (lane h) and with the immunogold technique on ultrathin cryosections an accumulation of hybrid protein in the cytoplasm is clearly shown (B). Bar = 0.2 jam. From [165],...

$Fig. 14. Localization of PhoE-LacZ hybrid proteins in Escherichia coli by means of cell fractionation studies (A) showing clearly the presence of the hybrid protein in the total cellular proteins of induced cells (lane e), the cell envelopes of induced cells (lane f) and the Triton X-100 insoluble protein fraction of the cell envelopes (lane h) and with the immunogold technique on ultrathin cryosections an accumulation of hybrid protein in the cytoplasm is clearly shown (B). Bar = 0.2 jam. From [165],...$

Takizawa T, Robinson JM (2003) Ultrathin cryosections an important tool for immimo-fluorescence and correlative microscopy. J Histochem Cytochem 51 707-714... [Pg.33]

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