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Ultrafast time-resolved fluorescence microscopes

The time-resolved techniques that are usually used for FLIM are based on electronic-basis detection methods such as the time-correlated single photon counting or streak camera. Therefore, the time resolution of the FLIM system has been limited by several tens of picoseconds. However, fluorescence microscopy has the potential to provide much more information if we can observe the fluorescence dynamics in a microscopic region with higher time resolution. Given this background, we developed two types of ultrafast time-resolved fluorescence microscopes, i.e., the femtosecond fluorescence up-conversion microscope and the... [Pg.68]

As described in the previous section, the femtosecond fluorescence up-conversion microscope enabled us to visualize microscopic samples based on position-depen-dent ultrafast fluorescence dynamics. However, in the imaging measurements using the fluorescence up-conversion microscope, XY scanning was necessary as when using FLIM systems. To achieve non-scanning measurements of time-resolved fluorescence images, we developed another time-resolved fluorescence microscope. [Pg.63]

See other pages where Ultrafast time-resolved fluorescence microscopes is mentioned: [Pg.52]    [Pg.52]    [Pg.69]    [Pg.537]    [Pg.537]    [Pg.134]    [Pg.100]    [Pg.221]   
See also in sourсe #XX -- [ Pg.51 , Pg.52 , Pg.53 , Pg.54 , Pg.55 , Pg.56 , Pg.57 , Pg.58 , Pg.59 , Pg.60 , Pg.61 , Pg.62 , Pg.63 , Pg.64 , Pg.65 , Pg.66 , Pg.67 , Pg.68 , Pg.69 ]



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Fluorescence microscopes

Microscopic time

Time-resolved fluorescence

Time-resolved fluorescence microscopes

Ultrafast

Ultrafast time-resolved fluorescence

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