Ultra fast gradient separation

Romanyshyn and Tiller [12] with ultra-short columns (20 mm x 2 mm, 10, 5 mm) and fast gradients were able to increase sample throughput and maintain the reliability of chemically different analytes during the chromatographic separations. Using this approach, the authors avoided matrix... 

The expected areas of future expansion of EFLC are in the separation of highly polar solutes. Mixtures of 61.7/27.7/10.6 and 55.7/25/1/19.2 mol ratio methanol/H20/C02 were predicted to have dielectric constants of 38 and 34 [28], respectively. Ion-exchange EFLC should be viable in these and other higher polar EFL mixtures, such as acetontrile/H20/C02 and THF/H2O/CO2 mixtures. Ultra high-speed gradient separations may be possible in EFLC as a result of the fast desorption rate constants for solutes under EFLC conditions. 

Figure 21.6. Ultra-performance liquid chromatography/MS/MS chromatograms for four compounds pooled, post-microsomal incubations, based on clogD. The four compounds are baseline separated using a 1.7-pm particles and a fast generic gradient.

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