Ubiquitin-specific proteases

Expressing recombinant proteins as N-terminal fusions with ubiquitin [109] is another strategy that can help to achieve proper folding. Additionally, this is an elegant way to obtain proteins that do not start with a methionine residue, since the ubiquitin moiety is cleaved off by endogenous ubiquitin-specific proteases [100]. 

Gewies, a. and Grimm, S. UBP41 is a proapoptotic ubiquitin-specific protease, Cancer Res, 2003, 63,... 

K., Miyazawa, S., Endo, S., and Suzuki, T. A novel ubiquitin-specific protease, synUSP, is localized at the post-synaptic density and post-synaptic lipid raft, J Neurochem, 2003, 87, 665-75. 

Baker, R. T., Tobias, J. W., and Varshavsky, A. Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family, J Biol Chem, 1992, 267, 23364-75. 

Compartment-Specific Functions of the Ubiquitin-Proteasome Pathway 2.3 Ubiquitin Specific Proteases... 

The ubiquitin marking system targets certain proteins for degradation. At least three enzymes, El, E2, and E3, are involved in addition to ubiquitin-specific proteases. 

Ubiquitin binding domain, which binds mono- or poly-ubiqitin Ubiquitin-specific protease, hydrolyzes both linear and branched Ub modifications Uridine diphosphate Uridine monophosphate... 

FIGURE 12.5 Partial view of a bioassay summary for a confirmatory (secondary screen) assay for ubiquitin-specific protease USP2a (AID 927). 

Everett RD, Meredith M, Orr A, Cross A, Kathoria M, Parkinson J (1997) A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein [corrected and republished article originally printed in EMBO J 1997 Feb 3 16(3) 566-577]. EMBO J 16 1519-1530... 

The split-ubiquitin system enables signal amplification from a transcription factor-mediated reporter readout (36,37). In one application, the interaction of two membrane proteins forces reconstitution of two halves of ubiquitin, leading to a cleavage event mediated by ubiquitin-specific proteases that release an artificial transcription factor to activate a reporter gene. As discussed, indirect readout of the reporter limits kinetic analysis, and the released transcription factor must translocate to the nucleus. 

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