Ubiquitin immunoreactivity

If neither p62 nor SMI-31 antibodies are available, ubiquitin immunoreactivity within muscle fibers can be used to differentiate s-IBM from polymyositis [14]. Ubiquitin-positive inclusions were also identifiable in formalin-fixed paraffin-embedded muscle biopsies of s-IBM patients, but not in any other inflammatory myopathies [15]. This is important for some laboratories when freshly frozen muscle biopsy specimens are not available (we do not know whether p62 antibodies detect PHFs in paraffin-embedded s-IBM sections). 

Figure 10.13 (a) and (b) XRF imaging of iron and zinc, respectively, of the temporal lobes tissue of the AD patient (ubiquitin immunoreactive). 

Figure 10.14 (a) and (b) XRF imaging of iron and zinc respectively of the temporal lobes tissue of the AD patient (poorly ubiquitin immunoreactive). The measurement area was 40 x 50 pm, and the step for each pixel is 1 pm. (c) Optical microscopic photograph of the sample stained with hema-toxylin-eosin after X-ray analysis. The scale bar is 20 pm. 2005 The Japan Society for Anal5dical Chemistry. 

Huang Y, Herman MM, Liu J, et al. 1997. Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer s disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates. Brain Res 771 213-220. 

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