Trypanothione synthetase

A third enzyme in the polyamine biosynthesis pathway, trypanothione synthetase-amidase (TRYS), has been shown to be essential by both genetic and chemical methods [24]. Specifically, the indazole analog 24 has been identified in a TRYS screen (IC50 = 140 nM) and shown to inhibit growth of wild-type (IC50 = 5.1 pM) and TRYS-dKO (IC50 = 0.46 mM) T. brucei parasites in culture [25]. 

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