Truncation suppression

The principal drawbacks to the technique are its labor-intensive nature and low protein yields, which in the best cases reach only a milligram or less. Competition with release factors at the amber stop codon often results in truncated protein as the primary product [16, 17]. Suppression efficiency rates are also affected by the character of the amino acid, which determines whether it is a good substrate for the ribosome and protein elongation factors. In addition, context effects variously ascribed to the influence of neighboring mRNA... 

Fig. 5. Comparison of suppression efficiencies of five tRNAs A in T4 lysozyme at site 82, and B in chorismate mutase at site 88. Suppression efficiencies are defined as the amount of full-length protein divided by the sum of the full-length and truncated protein produced in each reaction. The suppression efficiencies shown represent the average of two trials. The tRNAs are identified below each bar Y yeast, E E. coli T Tetrahymena rt readthrough (un-acylated tRNA) V acylated with valine hE acylated with homoglutamate. Reprinted with permission [33]...

This is a function of N and the N, (as well as the fixed control parameters II, T, which we suppress in our notation). We can therefore write the excess Gibbs free energy per particle, G(Ni,N)/N = g(mi), as a function of the moments m, = Nt/N (i = 1... K) of the normalized particle distribution n a). Altogether, die Gibbs free energy per particle of a truncatable system becomes... 

The third discharge stage is accompanied by current stoppage, separation of shock wave from the gas bubble and continuation of its expansion by its own momentum. The shock wave is suppressed by the surrounding liquid, and the pressure inside the bubble falls abruptly. Carbon structures take under such conditions the shape of a tore, truncated sphere, etc. 

Postprocessing suppression methods (see Postprocessing and selective detection-based methods, p. 325) have particular relevance to imaging since insufficient apodization and truncation can result in ringing over a large portion of the spectrum. This is often the case in spectroscopic imaging using multiple spin-echoes. ... 

Yau, K.W., and Nakatani, K. (1985) Light-suppressible, cyclic GMP-sensitive conductance in the plasma membrane of a truncated rod outer segment. Nature 317, 252-5. 

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