Triethanolamine cacodylate

Silvisar-510 (mixture of cacodylic acid and triethanolamine cacodylate) MALLARD, Anasplatyrhynchos Sodium arsenate... 

Reyes (1971) described a very sensitive assay for measuring the milkclotting activity of low concentrations of residual proteolytic enzymes in curd and whey. The substrate, buffered at pH 5.7, consisted of 1 g MSNF dissolved in a mixture of 70 ml 6.6 x 10 2 M cacodylic acid, 30 ml 6.6 x 10 2 M triethanolamine, and 1 ml 3 M CaCl2. 

We have found that certain commonly used buffers are incompatible with electron microscopy in general (Tris) or microscopy of nuclei in particular (HEPES, phosphate). Triethanolamine (TEA) has been widely used as a buffer for glutaraldehyde fixation of isolated chromatin (e.g., Thoma et ai, 1979), but is unsatisfactory for whole nuclei. Sodium cacodylate and PIPES are, in our experience, consistently reliable buffers. 

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