Trehalose formation

H. Bredereck, G. Hagelloch, and E. Hambsch, Chem. Ber., 87 (1954) 3 -37 the trehalose formation described in this paper may be interpreted accordingly. 

Trehalose accumulates as a byproduct during glucose-based L-lysine production by C. glutamicum. Overexpression of wild-type or mutated zwf decreased trehalose formation as consequence of an increased flux through the PPP [60]. The use of sucrose can also prevent the formation of certain byproducts at a L-lysine yield comparable to that on glucose, in contrast to fructose where the formation of lactate reduces L-lysine production by 30% [69]. Accumulation of lactate can be avoided by the deletion of the NAD-dependent L-lactate dehydrogenase gene IdhA [70]. 

Thomsen,133 Smolenski and Kozlowski,134 and Reeves and Blouin131 observed that sodium hydroxide has a relatively large effect on the optical rotation of sucrose. a,a-TrehaloSe,m on the other hand, is affected only slightly. Thomsen133 and Reeves and Blouin131 made no attempt to interpret the unusual behavior of sucrose Smolenski and Kozlowski,134 however, assumed that the reaction was that of alcoholate formation, and they calculated dissociation constants for sucrose. 

Very successful experiments were carried out on the oxidation of sucrose, one mole of which needs three moles of periodic acid, with the formation of one mole each of formic acid and a tetraaldehyde. The latter is oxidized by bromine to a tetracarboxylic acid subsequent acid hydrolysis of the oxygen bridges affords a mixture of acids, all of which were isolated and identified. These results brought in 1943 a confirmation of the structure of sucrose which was discussed, and which gained the approval of C. S. Hudson who, before that, was a little doubtful about the furanoid form of the D-fructosyl group. Similar work was done later with trehalose. In the same way, J. . Courtois obtained confirmation of the structure of raffinose, and established that of stachyose. 

Maruta, K., Nakada, T., Kubota, M., Chaen, H., Sugimoto, T., Kurimoto, M., and Tsujisaka. Y. 1995. Formation of trehalose from maltooligosaccharides by a novel enzymatic system. Biosci. Biotechnol. Biochem., 59,1829-1834. 

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