Transporter tools transfected cell lines

Applications of cationic dendrimers as transport vehicles have been described, with an emphasis on their use as transfection agents for nucleic acids. They can transfect them into a large number of cell lines and primary culture cells.Dendrimers with protonable amine groups can interact with all forms of nucleic acid by electrostatic interactions to form complexes that condense the nucleic acids. Whereas linear polymers often adopt random coil structures, the 3D structure of a dendrimer is characterized by radial symmetry. To solve many cellular obstacles of gene delivery, functionalization and variation of the dendrimer structure is an important tool that can be applied. These systems have shown stability and non-immunogenic properties for their potential use in a high variety of therapeutic applications and have been successfully used as carriers for nucleic acids and drug delivery. ... 

LLC-PK1 Cells derived from pig kidney epithelial cells (LLC-PK1) have been used as an alternative to Caco-2 cells for assessing the permeability for test compounds [153, 85], LLC-PK1 cells are more easily transfected than Caco-2 cells. Especially the LLC-PK1 line stably transfected with P-gp/MDRl has been reported as a tool to study bidirectional transport of compounds [142, 58, 180],... 

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