Transmission electron microscopy cryo-TEM

Won Y-Y, Brannan AK, Davis HT et al (2002) Cryogenic transmission electron microscopy (cryo-TEM) of micelles and vesicles formed in water by poly(ethylene oxide)-based block copolymers. J Phys Chem B 106 3354-3364... 

It is possible to observe the morphologies of the cationic PMETAI stars in solution by cryogenic transmission electron microscopy (cryo-TEM). Figure 1 clearly shows the distinct and well-separated structures in the vitrified solution. In some cases it is even possible to see the silsesquioxane core [28],... 

A technique called cryogenic temperature transmission electron microscopy (cryo-TEM) has been used by biologists for some years but is now also being used by chemists to examine a wide range of samples. CryoTEM involves the fast cooling of a thin film of liquid sample and transferring the sample into the TEM where it is maintained at... 

Cryogenic transmission electron microscopy (Cryo-TEM) or cryogenic high resolution transmission electron microscopy (Cryo-HRTEM) involves the observation of samples of vitrified macromolecules. 

As a complementary technique to SAXS, the cryogenic Transmission Electron Microscopy (cryo-TEM) enables the visualization of the shape of the dispersed particles and their internal nanostmctures. The right side of Figure 2... 

The various topologies of DNA (supercoiled, circular, linear) can be discriminated by various methods such as electrophoresis and by microscopy techniques such as electron microscopy (EM) [36], cryogenic transmission electron microscopy (cryo-TEM) [37], and atomic force microscopy (AFM) [38]. 

Vesicles can be characterized with the help of a range of physicochemical methods. Microscopy is an essential tool in all investigations of vesicles. MLVs and GUVs can be observed in real time by optical and fluorescence microscopy. LUVs and SUVs are best observed by transmission electron microscopy (TEM), ideally by stain-free methods such as cryogenic transmission electron microscopy (cryo-TEM), which can easily resolve the individual membrane secluding each vesicle. Depending on the stability of vesicles in the absence of solvent, vesicles can... 

Fig. 9 (a) Core-corona structure of IPECs formed by the star-shaped PANa fiilly charge-compensated with P2VPQ- -PEO. (b) Cryogenic transmission electron microscopy (cryo-TEM) image of the IPEC cores. Reprinted with kind permission from Springer Science + Business Media from [49] Copyright 2011, Pleiades Publishing, Ltd. 

Microemulsion structure can be observed by electron microscopy (EM). Jahn and Strey (4) were the first to publish reliable electron microscopy images of microemulsions. Their systematic study involved both droplet and bicontinuous structures, using the so-called freeze-fracture technique where a replica of the sample is made which is then monitored by the electron microscope. Another technique, Cryo transmission electron microscopy (Cryo-TEM), is probably less suitable for microemulsions with larger oil contents. In this technique, a thin slice of the sample is directly monitored... 

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