Transmembrane movements after

As the 26S mRNA is translated, the C protein is produced first and catalyzes its own cleavage from the nascent polypeptide soon after the ribosome transits into the sequences that encode E3. After release of the C protein, the free amino terminus of E3 is bound to the membranes of the rough endoplasmic reticulum. As the synthesis of nascent E3 and E2 continues, the polypeptide is translocated into the lumen of the endoplasmic reticulum, where oligosaccharides and fatty acids are added.78 A domain of hydrophobic amino acids near the carboxyl terminus of E2 inhibits further transmembranal movement so that the last 30 to 40 amino acids of the E2 polypeptide remain exposed on the cytoplasmic side of the membrane. It is thought that the 6K... 

Acid-released sycamore maple cell-wall fragments inhibit cellular growth. High fragment concentrations killed the cultured cells (50). At sublethal doses, wall fragments inhibited the transmembrane movement of amino acids within 1 minute after treatment. After 1 hour of treatment, amino acid transport was restored. Total protein synthesis was suppressed independently of the effect on amino acid transport. Results were related to the mechanism of killing plant cells by pectic enzymes and to the defense responses shown by plants to pathogens. 

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