Translocation Domains and Polyplexes

43] demonstrated that guanidinium-functionahzed PNBEs 49 and 50 were transported through eukaryotic. Using the length control afforded by ROMP, the authors determined that the optimum number of guanidinium repeats for 

In addition to covalently modified ROMP polymers, noncovalent poly-plexes of cationic ROMP polymers with DNA have been used as transfection agents [218, 219]. Breitenkamp and Emrick showed that PCOE functionalized with cationic polylysine grafts complexed with a plasmid that expresses GFP. This polyplex transfected COS-1 and HeLa cells at comparable or superior levels to commercially available jetPEI (linear polyethyleneimine), SuperFect (dendrimer), and Lipofectamine 2000 (liposomal carrier) transfection vehicles [220]. The ROMP-derived carrier was nontoxic to cells, while all three commercially available reagents led to significant levels of cell death. 

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