Translational control interferon-induced,

Several models, yet relatively little direct evidence has been presented to explain the genetic regulation of interferon production. No matter what model may be built to accomodate all known facts, it should be composed, in its simplest form, of an operon (interferon gene), a repressor protein, and a mRNA. The repressor protein may combine with the operator of the operon (referred to as transcriptional control in Fig. 7) in this state, the interferon gene would be repressed. It would be derepressed if the repressor is inactivated, e.g. following contact of the cell with the interferon inducer. The derepressed interferon gene would then be transcribed to mRNA, which in turn is translated to interferon. Hypothetically, the repressor protein may also combine with the mRNA for interferon (referred to as translational control), and even with the interferon molecule itself (referred to as posttranslational control in Fig. 7). 

Le Dinh T, Freneaux E, Labbe G, Letteron P, Degott C, Geneve J, Berson A, Larrey D, Pessayre D (1988) Amineptine, a tricyclic antidepressant, inhibits the mitochondrial oxidation of fatty acids and produces microvesicular steatosis of the liver in mice. J Pharmacol Exp Ther 247 745-750 Le Roy F, Bisbal C, Silhol M, Martinand C, Lebleu B, Salehzada T (2001) The 2-5A/RNase L/RNase inhibitor (RLl) pathway regulates mitochondrial mRNAs stability in interferon-a-treated H9 cells. J Biol Chem 276 48473 8482 Le Roy F, Silhol M, Salehzada T, Bisbal C (2007) Regulation of mitochondrial mRNA stability by RNase L is translation-dependent and controls IFNalpha-induced apoptosis. Cell Death Differ 14 1406-1413... 

Most of the cell-free extracts used in these studies had been pre-incubated to reduce the translation of endogenous mRNA. Since different preparations of lysates exhibited varying degrees of interferon-induced inhibition, we studied the influence of the length of time of pre-incubation on the interferon-induced inhibition of vitro protein synthesis. e could establish that the ability of cell-free extracts to translate mengo virus RNA was a function of the time of pre-incubation. The ability of an extract from interferon-treated (int. S-10) cells to translate mengo virus RNA was not impaired when compared with an extract from control cells until between 60 and 90 of pre-incubation. At this point, although the activity of the control extract declined somewhat, the activity of the Int. S-10 declined much more rapidly. 

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