Translation Inhibition by PK

Analysis of the cell-free translation system by sucrose gradient centrifugation, shows that PK-i causes a marked decrease in the amount of (55S-met-tRNA 4OS ribosome) complexes (12). Exogenous eIF-2 overcomes part of the dsRNA-dependent translational inhibition of mengo or globin mRNA in extracts from interferon treated cells (13)  

therefore, likely, that phosphorylation of eIF-2 impairs one of its function required for met-tRNA binding to 4OS ribosomes, as shown recently in reticulocyte lysate for the haemin-regulated eIF-2 phosphorylation (14, I5). A role for the other ribosome-associated proteins phosphorylated by the dsRNA-dependent protein kinase, e.g. protein 67 and I7 (4) the translational inhibition is, however, not excluded. Factor Ml may also be involved (5)  

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