Transfers from Liquid to Solid Media

Flame the loop to red-hot in an open flame. Cool the loop by carefully touching the surface of agar where no colonies can be seen. 

Select a colony from the Petri plate, slant, or stab culture. 

Pick up the test tube containing the sterile liquid medium with the other hand. 

Remove the test tube cap with the same hand that is holding the loop. Some microbiologists remove and keep caps between their ring and pinkie fingers on the backside of their hands so that the thumb and hand can hold the loop. 

Flame the test tube and immerse the loop into the liquid, rubbing the loop against the glass to assist in cell removal. 

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