Transferrin control experiments

Both parameters, the maximum intensity and time of the peak, were comparatively increased in EFA6 expressing cells demonstrating that EFA6 had a retention effect on occludin at the cell surface. In the control experiment, EFA6 had no effect on transferrin receptor cell surface delivery or half-life (Luton et al., 2004). While the calcium switch allows one to analyze the TJ assembly and disassembly, this method tests the stability of the TJ at steady state without the disturbance of the calcium switch. 

Spectroscopic data obtained from spectroelectrochemical experiments require careful and case-specific analysis. The Fe /Fe redox couple has a unique role in diflferent iron-containing proteins. It is hypothesised that the mammalian iron-transport protein transferrin uses the Fe /Fe redox couple as a switch that controls the time and site-specific release of iron, while other iron-containing proteins, such as myoglobin, are able to hold on to iron in both oxidation states. Therefore, it is very important to evaluate the protein and its interaction with both the oxidised and reduced states of iron and accordingly develop a data-analysis model. The spectroelectrochemical response of an iron binding protein can be ideal Nernstian, non-Nernstian resulting from coupled... 

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