Transfer reactions irreversible contact reaction

However, at still larger concentrations only DET/UT is capable of reaching the kinetic limit of the Stem-Volmer constant and the static limit of the reaction product distribution. On the other hand, this theory is intended for only irreversible reactions and does not have the matrix form adapted for consideration of multistage reactions. The latter is also valid for competing theories based on the superposition approximation or nonequilibrium statistical mechanics. Moreover, most of them address only the contact reactions (either reversible or irreversible). These limitations strongly restrict their application to real transfer reactions, carried out by distant rates, depending on the reactant and solvent parameters. On the other hand, these theories can be applied to reactions in one- and two-dimensional spaces where binary approximation is impossible and encounter theories inapplicable. 

The protein-coated electrode can be transferred sequentially between different electrolyte solutions, thus subjecting the redox centers to a range of chemical environments and opportunities for reaction. Conditions that can be examined even include extremes of pH, or other media such as cryosolvents—in which the protein would normally denature or undergo some other irreversible change during the time required for manipulations and measurements in conventional experiments [39]. By contrast, a typical cyclic voltammogram can be measured very quickly, thus allowing a snapshot to be taken before the sample deteriorates in practice this delay need be little more than a few seconds after the electrode has contacted the cell solution. 

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