Transfection polyclonal antibody

Figure 2 Recombinant expression of human mEH protects V79 Chinese hamster cells (V79 cells) from styrene-7,8-oxide-induced DNA single-strand breaks. (A) Western blot analysis of mEH transfected (lanes 1—6) and mock transfected (lane V) V79 cells. S9 fractions of the different clones were analyzed with a polyclonal antibody against purified human mEH raised in rabbits. Lane M prestained molecular weight markers (195, 112, 84, 63, 52, 35, and 32 kd). (73) mEH activity of the same clones using styrene-7,8-oxide as a substrate. Values are expressed as nanomoles of styrene glycol formed per minute and milligrams of protein. (C) Effect of styrene-7,8-oxide on DNA single-strand breaks using mEH transfected clone No. 3 [from (A) and (73)] and mock transfected V79 cells (7).

A wide selection of monoclonal and polyclonal anti-Ca -ATPase antibodies have become available in recent years. Studies with these antibodies defined the localization of Ca " -ATPase in the sarcoplasmic reticulum of developing and mature skeletal muscles [60,262-270] and established a pattern of cross reactivity with various Ca -ATPase isoenzymes in the sarco(endo)plasmic reticulum [270-286] and in the plasma membrane [284,287-290] of skeletal, cardiac and smooth muscles. Antibodies have also proved useful in the quantitation of Ca -ATPase, both in muscles of diverse fiber types [291-294] and in COS-1 cells transfected with Ca -ATPase cDNA [97,103,126,127,129,215],... 

---