Transfection nanoparticulate

Figure 22.3 Epithelial cells can be transfected to therapeutic protein to the basolateral target serve as secreting platform of the therapeutic compartment. This principle of circumventing protein. Protein as such does not permeate the apical tight junctions was demonstrated across the tissue boundary. The nanoparticulates recently in the eye [35]. DNA nanoparticulates that encode the protein also cannot permeate the were administered to the tear side of the cornea epithelial barrier, but they can internalize to the and the transgene product, SEAP, was secreted outermostall layerofthe epithelium. These cells to the anterior chamber of the eye. are transfected and subsequently secrete the...

Park et al. [60] prepared non-arginine-modified chitosan-based nanoparticulate systems for gene [small interfering RNA (siRNA)] deUvery. Modified chitosan-based nanoparticles resulted in increases in the transfection efficiency and ceUular uptake capacity (Fig. 10.5) as well as low cytotoxicity activity. 

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