Transendothelial chemotaxis assay

Figure 1 Schematic of a chemotaxis experiment. "A" shows a diffusion gradient assay, and "B" shows a transendothelial chemotaxis assay.

As seen in Fig. 1, two main modalities can be distinguished. Cell migration in diffusion gradient chemotaxis assays is mainly dependent on the strength of the chemokine-receptor interaction, but transendothelial... 

The cellular targets of MDC, with respect to chemotaxis, include monocyte-derived dendritic cells, IL-2-activated NK cells (maximal effect observed at 1 ng/mL MDC for both cell types), and activated T lymphocytes. It is important to note that whereas the former observations (responses of DC and NK cells) involved use of human cells in microchemotaxis assays (40), the latter observation was based on the ability of STCP-1 to stimulate transendothelial migration of activated murine T lymphocytes (41). In microchemotaxis assays, peripheral blood monocytes also appear to respond, but this effect requires significantly greater amounts of MDC (maximal effect at 100 ng/mL) than those for chemotaxis of DCs and NK cells. Whereas STCP-1 stimulated transendothelial migration of activated T cells, neutrophils, eosinophils, and peripheral blood monocytes failed to respond. 

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See also in sourсe #XX -- [ ]

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