Totipotency regeneration

The totipotency is the ability to generate or regenerate a whole organism from a part. 

Protoplasts are osmotically weak plant cells dispossessed of their cell wall, also referred to as naked cells. Upon removal of the cell wall, protoplast can behave like animal cells which by nature have no cell wall barrier. Protoplasts have the potential ability to redifferentiate into whole plants due to their totipotency, and this has been exploited for genetic modification of plants however, there exist many recalcitrant plant species which have not been able to be transformed following this method, either because of failure to isolate viable protoplasts or impossibility of regeneration. 

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