Total test approach immunohistochemistry

Taylor CR. The total test approach to standardization of immunohistochemistry. Arch. Pathol. Lab. Med. 2000 124 945-951. 

As described in Chapter 1, the test battery approach is a pretest to establish an optimal AR protocol, based on the fact that two major factors (heat and pH) influence the achievement of a satisfactory result of AR-immunohistochemistry (IHC). As indicated in Table 1.1, a total of nine slides are required to test three conditions of heating temperature, and three different pH values of the AR solutions. Practically, it may be performed in simpler ways as shown in Table 1.2. The following suggested protocol is based on our experience. 

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