Tobacco peroxidase conjugates

Surugiu et al. [67] have introduced an Enzyme Immuno-Like Assays (EzILA) for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The label was a 2,4-D conjugate with the tobacco peroxidase (TOP) enzyme, which allows for both colorimetric and chemiluminescent detection. In this case, the polymer imprinted with 2,4-D was synthesized in the form of microspheres. In contrast, despite its higher binding capacity for radiolabeled 2,4-D, a conventional MIP prepared by bulk polymerization showed only weak binding of the 2,4-D-TOP tracer. 

Recently, an enzyme-labelled assay for 2,4-D has been presented [19]. The label was 2,4-D conjugated to the enzyme tobacco peroxidase, which allowed for both colorimetric and chemiluminesence detection. In this instance, the 2,4-D MIP was synthesised in the form of microspheres [20]. In contrast, despite its higher binding... 

Having established the selectivity of the microspheres for 2,4-D, the template was then conjugated to tobacco peroxidase (TOP). Briefly, TOP was activated using NaI04 then coupled with diaminopropane. 2,4-D was converted to its N-hydroxysuccinimide ester and allowed to react with the derivatised TOP. After purification, the final 2,4-D/TOP ratio was estimated to be 8/1. 

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