Titration, immunochemical

A clear demonstration that aldolase is a mandatory catalyst in liver PPP involved the immunochemical evidence of Bleakely et al. (43) who showed the total cessation of hexose 6-P formation when liver aldolase antibody titrated the removal of aldolase from the system where Rib 5-P was reacted with the same rat liver enzyme preparation that established the Fig. 2 scheme. Irrespective of the other contrary data, this evidence alone showed another reaction mechanism involved Aid in liver PPP. The claim that aldolase is an essential enzyme in the PPP was also supported by data of (60) in which, using an in vitro construction of a PPP preparation for the complete oxidation of Glc, noted the formation of Oct-P and the need to include aldolase and sedoheptulose 1,7-bisphosphatase for the construction system to work. 

The aims of immunochemical analysis are the enumeration, identification, and eventually the titration of the various antigens contained in an unknown mixture. The concentration of antibodies in antisera against these antigens may also be determined. 

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