Tissues handling

Chung J-Y, Braunschweig T, Williams R, et al. Factors in tissue handling and processing that impact RNA obtained from formalin-fixed, paraffin-embedded tissue. J. Histochem. Cytochem. 2008 56 1033-1042. 

Both acetic anhydride and sulfuric acid are corrosive to tissue handle both with care and wipe up any spills immediately... 

Retrobulbar injections of radiolabeled methyl prednisolone acetate (insoluble) were found to produce elevated concentrations of steroid in the uvea, vitreous, and lens compared to IM injections on the squirrel monkey (56). This finding was not reproduced by Barry et al. (57), who also used radiolabeled methylprednisolone acetate, but failed to show a significant difference in either vitreal or uveal concentrations between treated and contralateral, untreated eyes after retrobulbar steroid injections in the albino rabbit. This apparent difference may have been due to interspecies variation or may be artifactual due to differences in tissue handling. 

Almeida, A., Thiery, J.P., Magdelenat, H., and Radvanyi, F. (2004) Gene exper-ession analysis by real-time reverse transcription polymerase chain reaction influence of tissue handling. Anal. Biochem. 328 101-108. 

Maxwell, RJ. Marmer, W.N. (1983) Lipids 18, 453-459, Systematic protocol for the accumulation of fatty acid data from multiple tissue samples tissue handling, lipid extraction and class separation, and capillary gas chromatographic analysis. 

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