Tissue culture laboratory

Doyle A, Griffiths JB, Newel DG (1994), Cell Tissue Culture Laboratory Procedures, John Wiley and Sons, New York, pp. 3 01 -3 03. 

Doyle A, Griffiths JB (1998), Cell and Tissue Culture Laboratory Procedures in Biotechnology, John Wiley Sons, Chichester, UK. 

Miller WM, Reddy A (1998), Quantitative analysis of cell growth, metabolism, and product formation, In Doyle A, Griffiths JB (Eds), Cell and Tissue Culture Laboratory Procedures in Biotechnology, John Wiley Sons, Guildford, UK, pp. 133-159. 

Honma M, Stacey G Mizusawa H (1993) DNA profiling with polymorphic DNA markers. In Cell and Tissue Culture Laboratory Procedures, pp. 9A 5.1-5.13. Wiley, Chichester. 

Because bovine serum (foetal, newborn, adult) remains fundamental to most tissue culture, it is still a necessity to batch test a lot prior to purchase. A reliable batch of serum is a major investment for any tissue culture laboratory. Assays based on the intended use should be in place (often there are more exacting requirements for hybridoma culture) and comparisons made between the material available from alternative suppliers. If a requirement for a particular project is the shipping of materials from one country to another, then the origin of the serum is also a consideration (e.g. US Department of Agriculture restriction on importation) (Stacey et al, 1998). 

Another area that has seen considerable advances is that of the physical environment for cell growth. Glassware has almost entirely disappeared from most tissue culture laboratories, to be replaced by an enormous, and expensive, range of plasticware. This has enabled the scientist to derive more sophisticated culture vessels and has led to the manufacture of three-dimensional culture systems and microcarriers. An added benefit has been the reliability and reproducibility of culture systems and, as a result, the avoidance of variability and contamination... 

Maroudas (1993) Physical and chemical treatments which affect cell adhesion. In Cell iSc Tissue Culture Laboratory Procedures, pp. 2E 2.1-2.11. Wiley, Chichester. 

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