Thyroglobulin immunogenicity

Since OVA and BSA possess some immunologically similar epitopes, a population of the antibodies produced against one often will cross-react against the other. Therefore, OVA cannot function as a non-relevant carrier for BSA and vice versa. Either OVA or BSA, however, may be used as non-relevant carriers for KLH, thyroglobulin, or the various toxoid proteins used as immunogen conjugates. 

Tg Polyclonal Tg-Ab Tg-fragments Autoantibodies Human serum IgG fraction was found to hydrolyse thyroglobulin (Tg). Unknown immunogen 3.9 X 1(T2 3.9 X 10 3 nr 5.7... 

To become immunogenic, a hapten has to be linked with a large molecule, such as a protein, prior to its introduction into the host animal. Several proteins can act as such carrier molecules including bovine serum albumin, human serum albumin, ovalbumin, thyroglobulin, poly-L-lysine, and hemocyanin. Among these proteins, bovine serum albumin is most commonly used because it is inexpensive, readily available, very soluble, highly immunogenic, and, in addition, resists denaturatlon (3). 

Continuous intraperitoneal infusion of insulin causes increased insulin immunogenicity, but it is not known if this is accompanied by an increased frequency of autoimmune diseases. Antibodies against insulin, thyroglobulin, thyroperoxidase, gastric parietal cells, smooth muscle,... 

To develop assays for d and 1-methadone, the hemisuccinates of a-l-methadol (Fig, 4 [4a]) were conjugated to bovine thyroglobulin (54). The resulting immunogens (Fig. 4 [4b]) caused formation of antisera that were quite selective when used with enantiomerically pure tritium-labeled d- or /-methadone. The cross-reaction of the I isomer with the d antiserum was less than 1%, and cross-reaction of /-methadone with the d antiserum was about 3%. The racemate exhibited cross-reactions of 56-57% (55). 

The method generally used for the preparation of the bile acid-protein conjugate is "mixed anhydride" technique described by Erlanger et al. or the carbodiimide method. Some authors have used thyroglobulin instead of bovine serum albumin as carrier protein of the immunogen because of the higher molecular weight. 

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