Thyroglobulin, antibodies

IMA and RIA are the two main methods used for the measurement of Tg. The IMA assays have the advantage of having a shorter incubation time and are automatable however, they suffer from greater interferences. The main interferants in both assays are antithyroglobulin antibodies, which cause an underestimation of Tg levels in the IMA. Antithyroglobulin antibodies can either be measured directly in all patients or if both IMA and RIA are used to measure Tg, a discordant result suggests the presence of anti-thyroglobulin antibodies. 

Wll. Watson, D., and Whinfrey, H., An electro-precipitin test for thyroglobulin antibodies. Lancet il, 1375 (1958). 

Chronic excessive iodine promoted the reactivity of anti-thyroglobulin antibody... 

Ohio State University Recombinant human thyroid-stimulating hormone Liothyronine Levo thyroxine Thyroglobulin Anti-thyroglobulin antibody Thyroid-stimulating hormone (Thyrotropin)... 

Some patients with lymphocytic thyroiditis have antibodies directed against the microsomal or cellular fractions only. Others have positive results for the thyroglobulin antibody, but have no antibodies against the microsomal fraction. The microsomal antibody is the most significant in relation to the etiology of thyroiditis as it is cytotoxic to cultured thyroid cells (D6). Microsomal autoantibodies are detected either by complement fixation technique (CFT) or by immunofluorescence. Be-... 

Function tests of thyroid activity have little or no value in establishing the diagnosis of lymphocytic thyroiditis because of the wide spectrum of thyroid activity that may be seen in this condition. Thyroid function is likely to be normal, low normal, or subnormal, but on occasions increased function may be encountered. The diagnosis of this condition rests on a clinical suspicion of its existence, the presence of microsomal or thyroglobulin antibodies at high dilution with purposefully insensitive techniques (as discussed in Section 4.3). Where indicated, the definitive diagnosis is made by biopsy. 

HI. Hackett, E., Beech, M., and Forbes, I. J., Thyroglobulin antibodies in patients without clinical disease of the thyroid gland. Lancet 2, 402—404 (1960). 

Affinity chromatography of human liver hydrolases Fractionation of low molecular weight porcine heparin into active and relatively inactive forms Affinity chromatography of heparin Affinity chromatography of rabbit anti-thyroglobulin antibodies Purification of Phaseolus vulgaris isolectins Purification of in vivo activity of an H-Z allo-antiserum... 

H.Tamaki, N.Amino, Y.Iwatani, F.Matsuzuka, K.Kuma,and K.Miyai, Detection of thyroid microsomal and thyroglobulin antibodies by new sensitive radioimmunoassay in Hashimoto s disease Comparison with conventional hemagglutination assay, Endocrinol Japon 38 97(1991)... 

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