Three-dimensional structures hydrogenase

Schematic drawing of the three-dimensional structure of D. gigas [NiFe] hydrogenase and close up on the active site... 

Matias, P. M., Soares, C. M., Saraiva, L. M., Coelho, R., Morais, J., LeGall, J. and Carrondo, M. A. (2001) [NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774 gene sequencing, three-dimensional structure determination and refinement at 1.8 angstrom and... 

Marques MC, Coelho R, de Lacey AL, Pereira IAC, Matias PM. The three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough a hydrogenase without a bridging ligand in the active site in its oxidised, as-isolated state. J Mol Biol. 2010 396(4) 893—907. 

The possible role of Ni in biology, the related biomimetic chemistry and the Ni-containing enzymes have been frequently reviewed in the last few years [15, 18, 24-28]. Here, we will concentrate on urase and hydrogenase, for which three-dimensional structures have recently become available [16, 17], and on CODH, for which a significant amount of spectroscopic data has been reported lately (see below). The various aspects of S-methylcoenzyme M reductase genetics, chemistry and biology have been very recently reviewed in this series [29] and will not be further addressed here. 

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