Thiol Modified Hyaluronic Acid

Thiol modified HA is generally prepared by conjugation with a disulfide containing molecules such as cystamine or a 3,3 -dithiopropionic acid dihydrazide that is coupled to HA using EDC chemistry. Reduction of disulfide bonds by dithiothreitol yields the desired thiol derivative. The thiol derivatives of HA has been extensively used to develop 

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Serban and Prestwich synthesised hyaluronic acid bromoacetate (HABA) by using bromoacetic anhydride [43]. The authors used this method to prepare crosslinker-free hydrogels by thiol alkylation using HABA as a polyvalent electrophile and thiol-modified HA (with or without thiol-modified gelatin) as a polyvalent nucleophile. 

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