Thiol detection

F. Ricci, F. Arduini, A. Amine, D. Moscone, and G. Palleschi, Characterisation of Prussian blue modified screen-printed electrodes for thiol detection. J. Electroanal. Chem. 563, 229—237 (2004). 

Figure 6 HPLC separation of a 4.0-nmol mixture of blood thiols detected by CL. (1) IV-acetyl-L-cysteine (2) reduced glutathion (3) cysteine (4) methionine (5) oxidized glu-tathion. (From Ref. 95, with permission.)...

C thiols detected in the "control slurry" (no tributylphosphine added) are interpreted as being in the unbound (or free) state thiols detected in "slurry with tributylphosphine" are interpreted as free plus bound species the thiol bound" was calculated from [(thiols in tributylphosphine treated slurry) - (thiols in control)] X 100%/(thiols in tributylphosphine treated slurry). 

This oxidation occurs at a potential at which very few other compounds are oxidized (+150 mV vs. Ag/AgCl). In the first application of this approach for CEEC, a 50-pm amalgamated gold wire was used in the off-column mode for the detection of glutathione in rat brain tissue homogenate [32], The run buffer and the samples were deoxygenated to avoid on-capiUary oxidation of the thiols. Detection limits for glutathione were 21 nM at S/N = 3. 

Piao, Yu., E. Shen, L. Han, and Sh. Cui. 2012. Reduced glutathione (GSH) and total thiol detection in some fruits by derivatizing methods. Shipin Gongye Keji 33(20) 60-64. 

Biolo cal/Medical Applications Analyzing/detecting of thiols detecting pathogen labeling amino acids producing MHC tetramers ... 

CgH ClIN M 255.485 Mediates a large number of synthetically useful transformns. (in the presence of base) particularly of carboxylic acids. Also acts as a thiol detecting agent. Yellow cryst. (Me CO). Mp 204-206°. Hygroscopic. 

Nekrassova et al. [13] have also studied the electrochemical oxidation of 5-thio-2-nitrobenzoic acid (TNBA) at a BDD electrode for the indirect detection of cysteine, homocysteine and glutathione. The reaction was shown to proceed via a CEC reaction process at lower pH, in which the thiol moiety of the TNBA species must undergo deprotonation before oxidation. These authors also reported that 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) can be used to develop the total thiol detection methodology using chronoamperometry with BDD electrodes. The detection limits of cysteine, homocysteine and glutathione were found to be 5.7 pM, 4.4 pM and 5.8 pM respectively. 

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