Thio thiosemicarbazides

Triazole-3(5)-thiol has been prepared by heating thio-semicarbazide and formic acid, by heating l-formyl-3-thiosemi-carbazide, and by heating 1,3,5-triazine and thiosemicarbazide. The ring closure of l-formyl-3-thiosemicarbazide using aqueous base was suggested by L. F. Audrieth and F. Hersman. 

Oxygen can be transferred from the arene oxides to nitrogen-, sulfur-, or phosphorus-containing substrates like pyridine, thiourea, N-methyl-benzothiazol-2-thione, thioacetamide, thiosemicarbazide, thiols, thio ethers, triphenylphosphine,21,160,161 etc. Parent hydrocarbons are formed as products.162 Thus the dimethoxycarbonyl oxide 269 on heating with pyridine produces 9,10-dimethoxycarbonylphenanthrene (270). The reaction of 1 or its... 

Another hydrazine derivative of fluorescein, 5-(((2-(Carbohydrazino)methyl)thio)-acetyl)-aminofluorescein, contains a longer spacer arm off its No. 5 carbon atom of its lower ring than fluorescein-5-thiosemicarbazide, described previously (Molecular Probes). The reagent can be used to react spontaneously with aldehyde- or ketone-containing molecules forming a hydrazone linkage (Fig. 209). It also can be used to label cytosine residues in DNA or RNA by use of the bisulfite activation procedure (Chapter 17, Section 2.1). The resulting fluorescent derivative exhibits a maximal excitation at 490 nm and a maximal luminescence emission peak at 516 nm when dissolved in buffer at pH 8. In the same buffered environment, the compound has an extinction coefficient of approximately 75,000 M-1cm 1 at 490 nm. 

The methods described in detail in Section 36.2, or only mentioned, have been used as follows for spectrophotometric determination of palladium the thio-Michler s ketone — in silver, copper, and anodic slime [32], in catalysts [31] with thiosemicarbazide derivatives — in water [44] and alloys [46] with palladium-carbon powder — with a-benzilmonoxime [48] with PAR — in catalysts and ores [58] with thiazolylazo derivatives — in Ni-Al catalysts [63] with 5-Br-PADAP — in titanium alloys with pyridylazo derivatives - in nickel alloys [68] with sulphonitrophenol - in silver alloys [70] with Arsenazo III — in iron and meteorites and with Palladiazo — in catalysts, minerals, silica gel, and calcium carbonate [78]. 

Compounds 40 react with guanidine, urea, thiourea, methylisothiourea, guanylthiourea by Lewis-acid catalysis (00S1738) and with thiosemicarbazide, semicarbazide (99IZV361) in the presence of triethylamine to form the corresponding 5,6-oligomethylenepyrimidines and 3-hydroxy-2(thio)-carbamoyl-3-polyfluor-o a 1 k y 1 - 3.3 a. 4.5.6.7 - h e x a h y d r o - 2 / /- i n d a z o 1 e s 41, respectively (Scheme 38). The structure of 41 (thio) is confirmed by X-ray analysis. 

A slow stream of ozonized oxygen passed 75 min. at -40° into a soln. of 1-acetyI-thymine in methanol, the resulting ozonide reduced with dimethyl sulfide in methanol during 12 hrs. at 0°, the crude mixture heated 20 min. at 80° with thiosemicarbazide, then treated 20 min. at 100° with 1 M NaOH 2-thio-6- azathymine. Overall Y 37%. F. e. s. M. Bobek, J. Farkas, and F. Sorm, Tetrah. Let. 1968, 1543. 

Synonyms N-Aminothiourea Hydrazinecarbothioamide micarbazide, thio- Semicarbazide, 3-thio- Thiocarbamoylhydrazine Thiocarbamylhydrazine 3-Thiosemicarbazide TSC... 

A soln. of 5-nitrofuran-2-carboxylic acid chloride in dioxane added dropwise to an ice-cooled suspension of an equimolar amount of 4-hydroxy-4-methyl-3-thio-semicarbazide and NaHCOg in dioxane containing some water with stirring, whidi is continued 3 hrs. at room temp. l-(5-nitro-2-furoyl)-4-hydroxy-4-methyl-3-thiosemicarbazide. Y 82%. F. e. and ring closure of the products s. E. Muller and P. Grobner, M. 106, 27 (1975). 

Thiosemicarbazides that are not substituted at the 4-position have been obtained by the action of ammonium rhodanide, or potassium rhodanide in the presence of hydrochloric acid, on hydrazines. A thio-semicarbazide of the type (210) was the only product obtained by the treatment of a 4-substituted thiosemicarbazide with diphenylphosphinyl chloride. ... 

Thiazolo[5,4-c]benzo(thio)pyrans.—[C3NS-C5X-C8]. Heterocyclic a-bromo-ketones (326) react with thioureas or thiosemicarbazides in alcoholic acetate-buffered solution to produce 2-substituted 4jEf-thiazolo[5,4-c]-benzo(thio)pyrans (327 X = O or S) in 30—55% yield. ... 

From thiosemicarbazides and thio)phosgene. Sasse has carefully reexamined the old-established synthesis of 1,3,4-thiadiazolines from substituted thiosemicarbazides and (thio)phosgene. 

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