Thin-layer electrochromatography

This review provides an overview of the literature published to date on macrocyclic antibiotics exploited for enantioselective separations in high-performance liquid chromatography (HPLC). It was not intended as a comprehensive issue on the applications of such antibiotics in sub- and supercritical fluid chromatography (SFC), thin layer chromatography (TLC), capillary electrophoresis (CE), and capillary electrochromatography (CEC). A number of structural properties of the most important macrocyclic antibiotics applied in HPLC enantioseparations are listed in Table 2.1. 

Pataki, G., Paper, thin-layer, and electrochromatography of amino acids in biological material, Z. Klin. Chem., 2, 129, 1964 Chem. Abs., 64, 5425c, 1966. 

Molecularly imprinted CSPs have been prepared for applications in HPLC, TLC (thin-layer chromatography), CE (capillary electrophoresis) and CEC (capillary electrochromatography). CSPs for HPLC are by far the most studied. 

Currently, most planar chromatography is based on the thin-layer technique, which is faster, has better resolution, and is more sensitive than its paper counterpart. This section is devoted to thin-layer methods. Capillary electrochromatography is described in Section 33D. 

A5. Ambert, J., Pechery, C., and Carpentier, C., Estimation of urinary amino acids. 1. In the normal subject. (Technique of electrophoresis at pH 5.4, two-dimensional chromatography on silica gel thin layers and electrochromatography.) Ann. Biol. Clin. (Paris) 24, 17-40 (1966). 

As mentioned above, GC and LC techniques (and GC-MS and LC-MS in particular) represent the major determinative approaches used in current MMRMs. Other, much less widely applicable and applied techniques include (1) capillary electrophoresis and capillary electrochromatography (2) thin-layer chromatography and (3) an array of immunoassays, such as immunosensors and enzyme-linked immunosorbent assay (ELISA). Immunoassays are, however, relatively useful for sensitive and rather rapid and inexpensive screening (followed by a confirmatory method in the case of a positive response) of selected pesticides for which ELISA kits or sensors are available. 

See also Capillary Electrochromatography. Capillary Electrophoresis Environmental Applications. Gas Chromatography Overview. Immunoassays Overview. Liquid Chromatography Overview. Mass Spectrometry Overview. Micellar Electrokinetic Chromatography. Sensors Overview. Spectrophotometry Ovenriew. Supercritical Fluid Chromatography Applications. Thin-Layer Chromatography Overview. 

Table 8.1 The chiral analysis of some environmental pollutants by micellar electrokinetic chromatography (MEKC), capillary electrochromatography (CEC) and thin layer chromatography (TEC)...

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