Thin layer chromatography ultracentrifuge

Fig- 2. Percent of total FAEEs in fractions obtained by density gradient ultracentrifugation. Pooled sera from 15 intoxicated emergency-room patients were subjected to density gradient ultracentrifugation and serum fractions were isolated. Lipids were extracted and FAEEs were isolated by thin-layer chromatography and quantitated by gas chromatography. (From Doyle 1994 with permission.)... 

The purification of glutamine cyclotransferase from papaya latex has been carried out by Messer and Ottesen (116). A batch procedure was used for the removal of impurities by passage of a papaya latex extract through a thin layer of carboxymethyl-Sephadex the active protein was separated by selective elution. Additional purification was achieved by chromatography on a column of carboxymethyl-Sephadex and by gel filtration on Sephadex G-100. The purified enzyme was homogeneous by the criteria of paper electrophoresis, ultracentrifugation, and gel filtration on Sephadex G-100 columns and chromatography on carboxy-methyl- or DEAE-Sephadex. The electrophoretic behavior of the enzyme indicates that it is a basic protein with an isoelectric point near... 

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