Thin-Layer Chromatography of Nucleoside Phosphates

The following solvents are suitable for chromatography of adenosine triphosphate (ATP) and derivatives  

Use a sheet of polyethyleneimine cellulose (PEI Cellulose), 50 x 200 mm, for chromatography. Mark the start about 15 mm away of the shorter side with a pencil. Apply the sample with a pipet or syringe. If larger volumes have to be used, apply them in several portions and/or a short line. 

Develop the chromatogram ascending in a chamber lined with filter paper and equilibrated with one of the solvents. 

After drying the sheet with a cold fan P-labeled nucleotides are identified by autoradiography or a Phospholmager. Unlabeled substances are identified by fluorescence quenching (see below). 

The most effective solvent is sodium hydrogenphosphate, if the detection is not done with phosphate reagent (e.g., Hanes reagent see below). 

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