Thin-layer chromatographic analysis toxicity

Further thin layer chromatographic analysis of the toxic fraction indicated a three component system with the major toxic component moving just off of the origin (Rf = 0.05) and two lightly detectable, non-toxic components occurring at Rf s 0.6 and 0.63. 

Thin layer chromatographic analysis is also highly applicable to the determination of aromatic organic acids.In human organisms, aromatic acids are synthesized as metabolites in intoxication by toluene, xylene, and ethyl benzene. These compounds are easily absorbed through the skin or respiratory system, and are oxidized to aromatic acids. The separation, identification, and quantitative analyses of aromatic acids are also necessary because they appear as semiproducts of the biosynthesis of aromatic amino acids in plants (phenolic acids), and metabolites of numerous toxic substances, drugs, and catecholamines. Polar adsorbents and polar-... 

M. E. Getz, Paper and Thin Layer Chromatographic Analysis of Environmental Toxicants, Heyden International Topics in Science Ser., 1981,1-175. 

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