Thick ascending limb cell culture

Glaudemans B et al (2014) A primary culture system of mouse thick ascending limb cells with preserved function and uromodulin processing. Pflugers Arch 466(2) 343-356... 

Tacrolimus may induce tubular dysfunction characterized as an increased excretion of urinary enzymes, decreased urinary concentrating ability, increased fractional excretion of magnesium in the presence of hypomagnesemia, hyperkalemia, hyperuricemia and fubular acidosis [12,260,645,647,705,736,737. In vitro sfudies showed fhat TAC inhibit Na/K - ATPase in rat microdissected cortical collecting duct and medullary thick ascending limb [738], and that high TAC doses added to primary human proximal tubules cultures decreased cell proliferation after 72 hours of incubation... 

Chang CT, Hung CC, Yang CW,Vandewalle A, Wu MS. Cyclosporine decreases prostaglandin E2 production in mouse medullary thick ascending limb cultured cells. TranspI Int 2005 18 871-878. 

PizzoniaJH, Gesek FA, Kennedy SM, Coutermarsh BA, Bacskai BJ, Friedman PA. Immunomagnetic separation, primary culture and characterization of cortical thick ascending limb plus distal convoluted tubule cells from mouse kidney. In vitro Cell Dev Biol 1991 27A 409-416. 

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